Comparing the Anti-oxidative Effects of "Three Decoctions for COVID-19"
10.13748/j.cnki.issn1007-7693.20223887
- VernacularTitle:“新冠三方”的体外抗氧化作用比较
- Author:
Ximeng LI
1
,
2
;
Yuan KANG
1
,
2
,
3
;
Yuan GAO
1
,
2
;
Zhuangzhuang LIU
1
,
2
;
Wenjing LI
1
,
2
;
Zhenlu XU
1
,
2
;
Xiaoyu ZHANG
1
,
2
;
Runlan CAI
1
,
2
;
Yun QI
1
,
2
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &
2. Peking Union Medical College, Beijing 100193, China
3. Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences &
- Publication Type:Journal Article
- Keywords:
Qingfei Paidu decoction； Huashi Baidu decoction； Xuanfei Baidu decoction； oxidative stress；COVID-19
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(7):887-894
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To compare the anti-oxidative effects of "three decoctions for COVID-19" (Qingfei Paidu decoction, Huashi Baidu decoction, Xuanfei Baidu decoction) in parallel experimental models.
METHODS
In the cell-free system, the total antioxidant capacity was investigated by FRAP method. The scavenging effects of DPPH radicals and superoxide anions were evaluated by DPPH and NBT reduction method, respectively. The scavenging effect of hydroxyl radicals was determined by a fluorescence method based on the end-product MDA. The anti-lipid peroxidation activity was investigated using the FeSO4-induced rat liver homogenate MDA method. Based on these five antioxidant indicators, the antioxidant capabilities of the extracts of three decoctions were parallelly compared in the cell-free system. Furthermore, in lipopolysaccharide-activated RAW264.7 cells, the productions of intracellular and mitochondrial reactive oxygen species(ROS) were detected using the L-012 probe and the MitoSOX mitochondrial superoxide red fluorescence probe, respectively; and intracellular NADPH oxidase activity was measured using the lucigenin probe. These three indicators were used to parallelly compare the antioxidant capabilities of the extracts of three decoctions.
RESULTS
In the cell-free system, three decoctions for COVID-19 could concentration-dependently scavenge DPPH radicals, superoxide anions and hydroxyl radicals, and potently inhibit the lipid peroxidation. At the equal extract concentration, their scavenging effects on DPPH radicals and superoxide anions and the total antioxidant capacity were comparable; while Huashi Baidu decoction exhibited the strongest ability to scavenge hydroxyl radicals and inhibit lipid peroxidation. In the cell system, three decoctions could reduce lipopolysaccharide-elevated intracellular ROS level by weakening NADPH oxidase activity; meanwhile, they could decrease mitochondrial ROS productions, among which Qingfei Paidu decoction possessed the most comprehensive effection.
CONCLUSION
Collectively, three decoctions for COVID-19 exert diverse antioxidant effects in both cell-free and cell systems, and each of them possesses the distinct advantages. Given that oxidative stress is pivotal during the pathological process of COVID-19, the results may suggest that the antioxidant ability of three decoctions is one of the pharmacodynamic basis for their clinical use.
