Rapid Identification of Chemical Components in Xitong Preparations by UPLC-Triple-TOF-MS/MS
10.13748/j.cnki.issn1007-7693.20224293
- VernacularTitle:UPLC-Triple-TOF-MS/MS快速鉴定豨桐制剂的化学成分
- Author:
Zonghan YANG
1
;
Guanqi TAO
1
;
Wenying SONG
1
;
Qixin ZHANG
1
;
Shifang XU
1
;
Wenkang HUANG
1
;
Yiping YE
1
Author Information
1. School of Pharmacology, Hangzhou Medical College, Hangzhou 310013, China
- Publication Type:Journal Article
- Keywords:
Xitong prepration ；UPLC-Triple-TOF-MS/MS；phenylpropanoids；terpenes；flavonoids；oxylipins
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(6):777-786
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish a rapid analytical method to identify the chemical components in Xitong preparations for the treatment of rheumatoid arthritis by ultra-high performance liquid chromatography coupled triple time-of-flight mass spectrometry(UPLC-Triple-TOF-MS/MS).
METHODS
The analysis was performed on an ACQUITY UPLC HSS T3 (2.1 mm×150 mm, 1.8 μm) column with the mobile phase consisting of water containing 0.1% formic acid(A) and acetonitrile containing 0.1% formic acid(B) in gradient mode at a flow rate of 0.3 mL·min−1. Data acquisition was carried out under positive and negative ion modes. The processed data was analyzed by Peakview software and compound database. The chemical components were determined by comparison with standard products, combining with the characteristic fragments in secondary MS, and those in the related literatures.
RESULTS
Seventy-eight compounds were identified including 20 phenylpropanoids, 33 terpenes, 19 flavonoids, 6 oxylipins, two of which might be new compounds.
CONCLUSION
The method can be used for rapid identification of chemical compositions in Xitong preparations, which provides basis for quality control and elucidation of phamarcodynamic material basis of Xitong pills and capsules .
