Effect of LncRNA OIP5-AS1 on Phenotypic Transformation of IOSE80 in Ovarian Epithelium
10.13748/j.cnki.issn1007-7693.20224213
- VernacularTitle:LncRNA OIP5-AS1对卵巢上皮IOSE80表型转化的影响
- Author:
Linlin SONG
1
;
Huanran MENG
1
;
Lina ZHOU
1
;
Rui LIU
1
;
Lijun YIN
2
Author Information
1. Department of Gynecology, The General Hospital of Ningxia Medical University, Yinchuan 750001, China
2. Department of General Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750001, China
- Publication Type:Journal Article
- Keywords:
ovarian epithelial cell ； lncRNA OIP5-AS1；cell cycle； phenotype
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(5):649-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To explore the phenotypic changes and possible mechanisms of lncRNA OIP5-AS1 on the proliferation, migration, apoptosis, invasion and cycle of ovarian epithelial cells IOSE80.
METHODS
The clinical data of patients were collected from TCGA database and GEO database. After R package analysis, the differential expression of OIP5-AS1 was visualized in the volcanic map. The correlation between survival rate and OIP5-AS1 was analyzed by Kaplan-Meier. The IOSE80 cell model of OIP5-AS1 over expression and silencing was constructed with lentivirus vector. The expression of OIP5-AS1 was verified by RT-qPCR. Cell proliferation was detected by CCK-8. Invasion was detected by Transwell. Cell migration was detected by scratch test. Cell cycle and apoptosis were detected by flow cytometry. Western blotting was used to detect the expression of E-cadherin and N-cadherin, as well as the expression of cyclin-dependent kinase(CDK) and cyclin-G-related kinase(GAK).
RESULTS
RT-qPCR results showed that IOSE80 cell lines over expressing and silencing OIP5-AS1 were successfully constructed. CCK-8 results showed that overexpressing OIP5-AS1 promoted the proliferation of IOSE80 cells. Scratch test results showed that overexpressing OIP5-AS1 promoted the migration of IOSE80 cells. Transwell results showed that overexpressing OIP5-AS1 would increase the invasiveness of IOSE80 cells. Flow cytometry results showed that overexpression of OIP5-AS1 weakened the apoptosis of IOSE80 cells and promoted the progress of cell cycle. Western blotting results showed that overexpression of OIP5-AS1 downregulated the expression of E-cadherin and upregulated the expression of N-cadherin, while overexpression of OIP5-AS1 increased the expression of CDK and GAK proteins.
CONCLUSION
LncRNA OIP5-AS1 further interferes with the regulation of IOSE80 cell cycle by up regulating the expression of CDK and GAK, and then indirectly regulates the malignant phenotype of ovarian epithelial cells.
