Effect of LncRNA OIP5-AS1 on Phenotypic Transformation of IOSE80 in Ovarian Epithelium
10.13748/j.cnki.issn1007-7693.20224213
- VernacularTitle:LncRNA OIP5-AS1对卵巢上皮IOSE80表型转化的影响
- Author:
Linlin SONG
1
;
Huanran MENG
1
;
Lina ZHOU
1
;
Rui LIU
1
;
Lijun YIN
2
,
3
Author Information
1. Department of Gynecology, The General Hospital of Ningxia Medical University, Yinchuan 750001, China
2. Department of General Surgery, People'
3. s Hospital of Ningxia Hui Autonomous Region, Yinchuan 750001, China
- Publication Type:Journal Article
- Keywords:
ovarian epithelial cell ； lncRNA OIP5-AS1；cell cycle； phenotype
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(5):649-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To explore the phenotypic changes and possible mechanisms of lncRNA OIP5-AS1 on the proliferation, migration, apoptosis, invasion and cycle of ovarian epithelial cells IOSE80.
METHODS
The clinical data of patients were collected from TCGA database and GEO database. After R package analysis, the differential expression of OIP5-AS1 was visualized in the volcanic map. The correlation between survival rate and OIP5-AS1 was analyzed by Kaplan-Meier. The IOSE80 cell model of OIP5-AS1 over expression and silencing was constructed with lentivirus vector. The expression of OIP5-AS1 was verified by RT-qPCR. Cell proliferation was detected by CCK-8. Invasion was detected by Transwell. Cell migration was detected by scratch test. Cell cycle and apoptosis were detected by flow cytometry. Western blotting was used to detect the expression of E-cadherin and N-cadherin, as well as the expression of cyclin-dependent kinase(CDK) and cyclin-G-related kinase(GAK).
RESULTS
RT-qPCR results showed that IOSE80 cell lines over expressing and silencing OIP5-AS1 were successfully constructed. CCK-8 results showed that overexpressing OIP5-AS1 promoted the proliferation of IOSE80 cells. Scratch test results showed that overexpressing OIP5-AS1 promoted the migration of IOSE80 cells. Transwell results showed that overexpressing OIP5-AS1 would increase the invasiveness of IOSE80 cells. Flow cytometry results showed that overexpression of OIP5-AS1 weakened the apoptosis of IOSE80 cells and promoted the progress of cell cycle. Western blotting results showed that overexpression of OIP5-AS1 downregulated the expression of E-cadherin and upregulated the expression of N-cadherin, while overexpression of OIP5-AS1 increased the expression of CDK and GAK proteins.
CONCLUSION
LncRNA OIP5-AS1 further interferes with the regulation of IOSE80 cell cycle by up regulating the expression of CDK and GAK, and then indirectly regulates the malignant phenotype of ovarian epithelial cells.
