Effect of Leech Combined with Whole Scorpion Lyophilized Powder on Carotid Artery Thrombosis, Coagulation Function and Fibrinolysis System in Rats
10.13748/j.cnki.issn1007-7693.20222461
- VernacularTitle:水蛭联合全蝎冻干粉对大鼠颈动脉血栓形成及凝血功能和纤溶系统的影响
- Author:
Qingsong WU
1
;
Shaohua JU
1
;
Ling ZHOU
1
;
Zhibin FU
1
;
Youli TAN
1
;
Ping LI
1
Author Information
1. Chengdu Sport University, Chengdu 610041, China；Sport Hospital Affiliated to Chengdu Sport University, Chengdu 610041, China
- Publication Type:Journal Article
- Keywords:
leech ； scorpion ； freeze-dried powder；thrombosis；coagulation function；fibrinolytic system
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(5):644-648
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To investigate the effect of leech freeze-dried powder combined with scorpion freeze-dried powder on carotid artery thrombosis in rats.
METHODS
FeCl3 was used to induce carotid artery thrombosis, and the experiment was divided into 6 groups: sham-operated group(sham group), model group, leech freeze-dried powder group(leech group), whole scorpion freeze-dried powder group(scorpion group), leech+scorpion half-dose group(half-dose group), leech+scorpion full-dose group(full-dose group), 6 rats in each group. The inhibition rate of bilateral carotid artery thrombosis was calculated after the final administration. The activated partial thromboplastin time(APTT), plasma prothrombintime(PT), plasma fibrinogen(FIB), and prothrombin time(TT) were measured by platelet aggregation and coagulation factor analyzer. The levels of plasma tissue-type plasminogen activator(t-PA) and plasminogen activatorinhibitor-I(PAI-I) were measured by ELSIA, and the thrombosis of each group was observed by HE staining.
RESULTS
Compared with model group, the wet weight and dry weight of thrombus were reduced in the leech, scorpion, half-dose and full-dose groups, and the full-dose group had the best effect in inhibiting thrombus formation. Compared with sham group, APTT, PT, TT was shortened, FIB content increased and t-PA level decreased in model group; compared with model group, APTT, PT, TT was prolonged, FIB content decreased and t-PA level increased in each dose group. The PAI-Ⅰ was not significantly different in each group. A large number of thrombus were seen in the blood vessels of model group. Compared with model group, there were significantly fewer thrombus in the leech, scorpion and half-dose groups, and almost no significant thrombus in the full-dose group.
CONCLUSION
Leech freeze-dried powder combined with scorpion freeze-dried powder inhibited carotid artery thrombosis in rats by affecting coagulation function and fibrinolytic system.
