HPLC Fingerprint and Content Determination of Five Components in Ranunculus Sceleratus L.
10.13748/j.cnki.issn1007-7693.20223192
- VernacularTitle:石龙芮HPLC指纹图谱及5种成分含量测定
- Author:
Shanshan CAO
1
;
Zhen SHI
1
;
Xiaowen ZHENG
1
;
Fei CHEN
1
;
Sijia ZHOU
1
;
Wenming CHENG
1
;
Yazhong ZHANG
2
;
Jin XIE
1
;
Qunlin ZHANG
1
Author Information
1. School of Pharmacy, Anhui Medical University, Hefei 230032, China
2. Anhui Institute for Food and Drug Control, Hefei 230051, China
- Publication Type:Journal Article
- Keywords:
Ranunculus sceleratus L. ； HPLC； fingerprint ； content determination ； quality evaluation
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(1):106-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish the HPLC fingerprint and content determination of five components in Ranunculus sceleratus L..
METHODS
The separation was developed on an Agilent ZORBAX SB C18 chromatographic (4.6 mm×250 mm, 5 μm)column by gradient elution with methanol(A)-0.1 % phosphoric acid aqueous solution(B) as mobile phase to establish HPLC fingerprint of Ranunculus sceleratus L.. Combined with similarity evaluation, cluster analysis, principal component analysis, and orthogonal partial least squares-discriminant analysis, the quality of 13 batches of Ranunculus sceleratus L. was evaluated.
RESULTS
Thirteen batches of Ranunculus sceleratus L. samples were calibrated with 20 common peaks, of which 5 common peaks were identified, and the similarity ranged from 0.874 to 0.984. The results of cluster analysis and principal component analysis were basically the same, indicating that there might be differences in the content of chemical components of Ranunculus sceleratus L. in different regions. Protocatechuic aldehyde, caffeic acid, ferulic acid, hyperoside and isoquercitrin were determined in thirteen batches of Ranunculus sceleratus L., and their contents were 0.016−0.035, 0.010−0.070, 0.010−0.029, 0.016−0.051, 0.028−0.086 mg·g–1, respectively.
CONCLUSION
The established HPLC fingerprint and content determination method is simple, stable, and reliable, which can be used for qualitative analysis and provide reference to quality evaluation and resource utilization of Ranunculus sceleratus L..
