Comparison of Anti-ulcerative Colitis Effect of Huangqin Decoction Combined with Paeoniae Radix Alba and Paeoniae Radix Rubra

Huajie HU; Xinyu Qiu; Jiayan Jiang; Yingying Wang; Liping Han; Zhaohuan Lou

Return

Comparison of Anti-ulcerative Colitis Effect of Huangqin Decoction Combined with Paeoniae Radix Alba and Paeoniae Radix Rubra

VernacularTitle:黄芩汤配伍使用白芍、赤芍抗溃疡性结肠炎作用比较
Author: Huajie HU ¹ ; Xinyu QIU ^{2
,

3} ; Jiayan JIANG ⁴ ; Yingying WANG ⁴ ; Liping HAN ⁴ ; Zhaohuan LOU ⁴
Author Information

1. Zhoushan Hospital of Traditional Chinese Medicine, Zhoushan 316000, China
2. School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China；Department of Pharmacy, Ningbo Women and Children&rsquo
3. s Hospital, Ningbo 315012, China
4. School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China
Publication Type:Journal Article
Keywords: Huangqin decoction ； Paeoniae Radix Alba ； Paeoniae Radix Rubra ； network pharmacology ； ulcerative colitis ； Notch signaling pathway
From: Chinese Journal of Modern Applied Pharmacy 2024;41(4):524-532
CountryChina
Language:Chinese
Abstract: OBJECTIVE :To study the difference of Huangqin decoction combined with Paeoniae Radix Alba(BS) and Paeoniae Radix Rubra(CS)'s effect on ulcerative colitis(UC) based on network pharmacological analysis and animal experiment. METHODS The active constituents of BS and CS were retrieved from TCMSP database and literature, and the potential target was predicted by Swiss Target Prediction. Ulcerative Colitis was used as key words to search disease targets in DisGenet, OMIM, and Genecard databases. The intersection target was obtained by Venny 2.1.0. Cytoscape 3.7.2 software was used to construct network of drug-consumption targets. The STRING platform was used for protein-protein interaction(PPI) network analysis, and Metascape database was applied for GO/WIKI analysis. A dextran sulfate sodium(DSS) induced UC mouse model was established to compare the anti-UC effects of Huangqing decoction combined with BS(HQT-BS) and CS(HQT-CS), respectively. RESULTS There were 7 active components of HQT-BS and 11 active components of HQT-CS in the treatment of ulcerative colitis, respectively, 5 of which were the same. There were 146 and 157 targets respectively, 106 of which were the same. The core targets of HQT-BS were SRC, HSP90AA1, and PIK3R1, while the core targets of HQT-CS were SRC, HSP90AA1, and STAT3. WIKI enrichment analysis showed that several signaling pathways were shared by both BS and CS, such as EGFR tyrosine kinase inhibitor resistance, Notch signaling pathway. EGF/EGFR signaling pathway was the specific pathway related to BS, while Nuclear receptors meta-pathway and Kit receptor signaling pathway were the specific pathways related to CS, respectively. Animal experiments showed that both HQT-BS and HQT-CS could significantly improve colon shortening and tissue pathological alternation induced by DSS. However, HQT-CS was more effective in reducing the expression of interleukin-6 and neurogenic locus notch homolog protein1. CONCLUSION Both HQT-BS and HQT-CS have anti-UC effect, and HQT-CS is the better one.