Quality Standard and Acute Toxicity Study of Triadica Cochinchinensis
10.13748/j.cnki.issn1007-7693.20224281
- VernacularTitle:山乌桕质量标准及急性毒性研究
- Author:
Fang LYU
1
;
Xiao XU
2
;
Xiaopeng WU
2
;
Yan YOU
3
;
Dongjie SHAN
1
;
Xueyang REN
1
;
Xianxian LI
1
;
Qingyue DENG
1
;
Yingyu HE
1
;
Gaimei SHE
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100029, China
2. Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
3. Guilin Hospital of Traditional Chinese Medicine, Guilin 541002, China
- Publication Type:Journal Article
- Keywords:
Triadica cochinchinensis； quality standard ； ICP-MS ； heavy metal； acute toxicity
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(4):512-519
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish the quality standard of Triadica cochinchinensis and to perform the acute toxicity study.
METHODS
Appearance properties, powder microscopic identification, and thin-layer chromatography(TLC) identification were researched. The specific chromatogram was established by HPLC. The content of cadmium(Cd), lead(Pb), arsenic(As), copper(Cu), and mercury(Hg) was determined by inductively coupled plasma-mass spectrometry(ICP-MS). Acute toxicity was studied by maximum dose.
RESULTS
The outer skin of herbs was dark brown, and the inner surface was light yellow brown and fibrous. Besides, crystal sheath fiber was common, and calcium oxalate clusters arranges in rows. In the TLC diagram of the test product, the fluorescent spots of the same color were displayed at the corresponding position of the control product(scopoletin, isofraxidin). Five common peaks were calibrated in the characteristic map and the three characteristic peaks(scopoletin, isofraxidin, dimethylfraxetin) were recognized. The content of the measured heavy metal elements was lower than the national limit standard. The linear correlation coefficient was R2 > 0.999. The precision, stability, repetitive RSD were < 10%. The average recovery rate of the added sample was 80%−120%, and the RSD was < 10%. The maximum dose of the acute toxicity test was 184.09 g·kg−1. The 14 d internal body mass, food intake, organ-body ratios, the serum glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, blood urea nitrogen, and creatinine were not significantly different by comparing with the normal controls. Therefore, no significant toxicity was observed.
CONCLUSION
The established standard can provide a reference for evaluating the quality of Triadica cochinchinensis. The heavy metal content of ten batches of medicinal materials is within the safe range. Acute toxicity test show that there is no obvious significant adverse teactions after oral administration, and the safe dose range is large, which can provide a reference for the subsequent development and utilization.
