Determination and Comparison of 26 Components in Different Parts of Two Base Plants of Shiliang Tea Based on UHPLC-MS/MS
10.13748/j.cnki.issn1007-7693.20230992
- VernacularTitle:基于UHPLC-MS/MS的食凉茶2种基原植物不同部位中26个成分的含量对比分析
- Author:
Juhua MAO
1
;
Junjie PAN
2
;
Zhangjin CHEN
1
;
Chaqing WU
1
;
Weiying WANG
3
;
Kejun CHENG
2
Author Information
1. Lishui Institute for Quality Inspection and Testing, Lishui 323000, China
2. Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, China
3. Lishui Institute for Quality Inspection and Testing, Lishui 323000, China；Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:Journal Article
- Keywords:
Shiliang tea ； Chimonanthus salicifolius S.Y. Hu ； Chimonanthus zhejiangensis M.C.Liu ； different parts； roots ； stems ； leaves； cluster analysis ； OPLS-DA ； UHPLC-MS/MS
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(4):489-495
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish an ultra-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method for determination and comparison of 26 components in different parts of two base plants of Shiliang tea(Chimonanthus salicifolius S.Y.Hu and Chimonanthus zhejiangensis M.C.Liu), and screen quality markers of different parts.
METHODS
The UHPLC method was performed on an Agilent RRHD Eclipse Plus C18 (2.1 mm×50 mm, 1.8 μm) column with a gradient elution of methanol and 0.1% formic acid in water at a flow rate of 0.3 mL·min−1, the column temperature was 35 ℃, and the injection volume was 0.5 μL; the multiple reaction monitoring mode was employed for the quantification of 26 components with electrospray ionization(ESI) source polarity in negative and positive mode.
RESULTS
Good linear relationship(r >0.999) were observed in the test ranges for 26 compounds, and the average recovery was 88.5%−111.7% with RSD was 3.4%−9.8%. There was no significant difference between the two base plants of Shiliang tea, and all of these samples were divided into two categories by hierarchical cluster analysis. The main components in leaves was flavonoids, among them, the content of kaempferol 3-O-rutinoside was the highest, reaching 12.902 mg.g−1; the main components in stems and roots was coumarins, and the content of alkaloids in roots was higher, relatively; 7 quality markers of difference were screened by OPLS-DA, which were kaempferol 3-O-rutinoside, chimonanthine, rutin, fraxetin, calycanthoside, scopolin, neochlorogenic acid.
CONCLUSION
These study elucidates the differences of chemical components in the different parts of two base plants of Shiliang tea, which providing basis for the research of pharmacodynamic substances and references for the comprehensive utilization of Chimonanthus salicifolius S.Y. Hu and Chimonanthus zhejiangensis M.C.Liu resources.
