Quality Evaluation of Traditional Chinese Medicine Jieze Lotion Based on Combination of Fingerprints and Multi-component Quantitative Analysis
10.13748/j.cnki.issn1007-7693.20230767
- VernacularTitle:基于指纹图谱和多组分定量分析的中药制剂洁泽洗液质量评价
- Author:
Yuheng BA
1
;
Qiulan WANG
1
;
Qing WAN
1
;
Wenqing WANG
1
;
Chunyang SHI
1
;
Zhuo CHEN
2
;
Jianguo FANG
1
Author Information
1. Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China
2. Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China
- Publication Type:Journal Article
- Keywords:
Jieze lotion ； HPLC ； fingerprints；multi-index determination ；chemometrics ； quality evaluation
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(4):452-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish fingerprints and multi-components determination of Jieze lotion, and use chemometrics methods for quality evaluation.
METHODS
The HPLC-DAD fingerprints was established and 10 components were recognized by comparison with references. Meanwhile, their contents were determined. The data were evaluated by the methods of chemometrics such as similarity evaluation, cluster analysis, principal component analysis, and orthogonal partial least squares-discriminant analysis.
RESULTS
The similarity of 11 batches of Jieze lotion were all >0.95. The linearity was good(r≥0. 999 1) and the average recoveries were between 89.70% and 106.0% with the RSD of 1.52%−3.41%. Instrument precision, stability and reproducibility of the method were all great. The contents of the common ten components(gallic acid, protocatechuic acid, neochlorogenic acid, caftaricacid, 5-O-feruloylquinicacid, chlorogenic acid, phellodendrine chloride, magnoflorine, 4-O-feruloylquinic acid, berberinehydrochloride) were 40.103−55.841, 2.347−6.179, 8.336−23.810, 7.084−21.956, 33.098−53.833, 24.597−49.610, 21.587−31.188, 5.915−13.162, 115.381−189.702, 31.378−112.686 μg·mL−1, respectively. The results of chemometrics showed that the 11 batches of samples could be divided into 4 categories, and the strong characteristic peaks used to distinguish each batch of samples were berberine hydrochloride, 4-O-feruloylquinic acid, chlorogenic acid, neochlorogenic acid and 5-O-feruloylquinic acid.
CONCLUSION
The method is accurate and reliable, and it can be used for the quality control and comprehensive evaluation of Jieze lotion.
