Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion.
10.14791/btrt.2014.2.1.22
- Author:
Jian PEI
1
;
Kyung Sub MOON
;
Sango PAN
;
Kyung Hwa LEE
;
Hyang Hwa RYU
;
Tae Young JUNG
;
In Young KIM
;
Woo Yeol JANG
;
Chae Hun JUNG
;
Shin JUNG
Author Information
1. Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea. moonks@chonnam.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Cathepsin D;
Gliomas;
Invasion;
Motility;
Protein;
Proteomics
- MeSH:
Cathepsin D;
Cell Line*;
Electrophoresis, Gel, Two-Dimensional;
Glioma*;
Humans;
Mass Spectrometry;
Proteomics
- From:Brain Tumor Research and Treatment
2014;2(1):22-28
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments. METHODS: High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed. RESULTS: Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A. CONCLUSION: Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
