Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China
- Author:
Xujie DUAN
1
;
Xiaona SHI
;
Pei ZHANG
;
Xiaoyue DU
;
Sixu CHEN
;
Liang ZHANG
;
Huiping LI
;
Yufei ZHANG
;
Jinling WANG
;
Yulin DING
;
Shuying LIU
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(5):e61-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:To investigate the pathological changes and conduct viral gene analysis of OPA and MVD co-occurrence in Inner Mongolia, China.
Methods:Using gross pathology, histopathology, immunohistochemistry, ultrastructural pathology, PCR, and sequence analysis, we investigated the concurrent infection of JSRV and MVV in 319 Dorper rams slaughtered in a private slaughterhouse in Inner Mongolia, in 2022.
Results:Of the 319 rams included, 3 showed concurrent JSRV and MVV infection. Gross lung pathology showed diffuse enlargement, consolidation, and greyish-white miliary nodules on the lung surface; the trachea was filled with a white foamy fluid; hilar and mediastinal lymph nodes were significantly enlarged. Histopathology results revealed typical OPA and MVD lesions in the lung tissue. Immunohistochemical results were positive for JSRV envelope protein (Env) in the tumor cells and MVV CA in alveolar macrophages. Transmission electron microscopy showed several virions and autophagosomes in the lung tissue, severely damaged mitochondria, and the induced mitophagy. Nucleotide sequences obtained for JSRV env and MVV gag showed the highest homology with the Inner Mongolian strains of JSRV env (JQ837489) and MVV gag (MW248464).
Conclusions:and Relevance: Our study confirmed that OPA and MVD co-occurrence and identified the pathological changes in Inner Mongolia, China, thereby providing references for the identification of concurrent JSRV and MVV infections.
