Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea
- Author:
Jeong-Byoung CHAE
1
;
Seung-Uk SHIN
;
Serim KIM
;
Hansong CHAE
;
Won Gyeong KIM
;
Joon-Seok CHAE
;
Hyuk SONG
;
Jung-Won KANG
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(5):e59-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:To identify, for the first time, the prevalence and impact of Boosepivirus (BooV) on calf diarrhea in the ROK.
Methods:Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (Bos taurus coreanae) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.
Results:The complete genome of BooV was detected from unknown causes of calf diarrhea.And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.
Conclusions:and Relevance: This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.
