Effect of Uteroglobin on cPLA2, COX-2 Expression and ERK Activation in NSCLC Cells.
10.4046/trd.2004.56.6.638
- Author:
Woo Jin KIM
1
;
Jung Min YOON
;
Kyoung Hee LEE
;
Seon Jin HAN
;
Won Hyuk SHIN
;
Jae Joon YIM
;
Chul Gyu YOO
;
Choon Taek LEE
;
Sung Koo HAN
;
Young Soo SHIM
;
Young Whan KIM
Author Information
1. Department of Internal Medicine and Lung Institute of Medical Research Center, Seoul National University College of Medicine, Seoul, Korea. ywkim@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Uteroglobin;
Lung cancer;
cPLA2;
COX-2;
ERK
- MeSH:
Blotting, Western;
Carcinoma, Non-Small-Cell Lung;
Cell Line;
Epithelium;
Inflammation;
Lung Neoplasms;
Phenotype;
Phosphotransferases;
Uteroglobin*
- From:Tuberculosis and Respiratory Diseases
2004;56(6):638-645
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Uteroglobin is a protein produced by the normal bronchial epithelium and its expression level is lower in non-small cell lung cancer tissues and cell lines. It mainly functions as an anti-inflammatory, and when it is overexpressed in cancer cells, the neoplastic phenotype is antagonized. cPLA2 and COX-2, which are also associated with inflammation, were reported to be related to cancer. The relationship between cPLA2, COX-2 and uteroglobin is unclear. The relationship between uteroglobin and ERK, which is related to cell growth, is also not unclear. This study investigated the changes in the cPLA2 and COX-2 expression levels and the ERK activities after the overexpression of uteroglobin in non-small cell lung cancer cell lines. METHODS: The A549 and NCI-H460 cell lines were infected by adenovirus-null and adenovirus- uteroglobin. The cChange in the cPLA2, COX-2 expression level and ERK activity after uteroglobin overexpression was measured by Western blot. The change in MMP activity was measured by zymography. RESULTS: Western blot revealed decreased expression levels of cPLA2, and COX-2, and increased pERK levels in nonsmall cell lung cancer cells after uteroglobin overexpression. Zymography revealed no changes in the MMP-2 activity and lower MMP-9 activity. U0126, which is a specific inhibitor of ERK-activating kinase MEK-1/-2, prevented the decrease in the MMP-9 activity CONCLUSIONS: A decrease in cPLA2 expression, COX-2 expression, MMP-9 activity and a increase in ERK activity may be related to the anticancer effects of uteroglobin in nonsmall cell lung cancer cells.
