The Effect of the Remnants after Cataract Extraction on the Lens Epithelial Cell Culture.
- Author:
Song Jun DO
1
;
Sug Jae KANG
;
Jae Hwan LEE
;
Jung Hyub OH
;
Jun Hee LEE
Author Information
1. MANUBASE Eye Clinic, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
In vitro capsular bag model;
Lens epithelial cell;
Posterior capsular opacity
- MeSH:
Capsules;
Cataract Extraction*;
Cataract*;
Cell Culture Techniques;
Epithelial Cells*;
Organ Culture Techniques;
Polymethyl Methacrylate
- From:Journal of the Korean Ophthalmological Society
2002;43(5):872-882
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To assess the effect of the remnants after lens extraction on posterior capsular opacification with lens epithelial cell culture through in vitro capsular bag model. METHODS: After isolating porcine lens capsules, sterile non-toxic PMMA (polymethyl- mathacrylate) tension ring was inserted into the capsule. These were placed in organ culture medium up to 6 weeks. The grade of cell coverage of the posterior lens capsule was recorded to check the proliferative activity. RESULTS: In the process of cell culture, outgrowth of the epithelial cells was observed across the posterior capsule after a lag period. The rate of cell coverage was dependent upon the added factors. The proliferative activity was the greatest in the group where lens cortical and nuclear materials were added, and other groups showed no difference from a control group. CONCLUSIONS: To reduce posterior capsular opacification, it is important that we should not leave the lens cortical material behind during cataract surgery.
