N-glycoproteomic analysis of human follicular fluid during natural and stimulated cycles in patients undergoing in vitro fertilization.
10.5653/cerm.2017.44.2.63
- Author:
Hee Joung LIM
1
;
Ae Eun SEOK
;
Jiyou HAN
;
Jiyeong LEE
;
Sungeun LEE
;
Hee Gyoo KANG
;
Byung Heun CHA
;
Yunseok YANG
Author Information
1. Forensic Science R&D Lab, Police Science Institute, Asan, Korea.
- Publication Type:Original Article
- Keywords:
Fertilization in vitro;
Human follicular fluid;
Hyper stimulation;
N-glycoprotein;
Natural cycle;
Proteomics
- MeSH:
Chemistry;
Complement System Proteins;
Consensus Sequence;
Female;
Fertilization in Vitro*;
Follicular Fluid*;
Humans*;
In Vitro Techniques*;
Infertility;
Mass Spectrometry;
Ovarian Hyperstimulation Syndrome;
Ovulation Induction;
Physiology;
Proteome;
Proteomics;
Reproduction;
Sensitivity and Specificity;
Thyroxine-Binding Globulin;
Up-Regulation;
Vitamin D-Binding Protein
- From:Clinical and Experimental Reproductive Medicine
2017;44(2):63-72
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. METHODS: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. RESULTS: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. CONCLUSION: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.
