Serum Level of TNF-alpha and Soluble TNF Receptor I in Infants with RDS and Their Significance as a Prospective Indicator for Development of Infantile Asthma.
- Author:
Bok Yang PYUN
1
;
Woo Ryoung LEE
Author Information
1. Department of Pediatrics, College of Medicine, Soonchunhyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
RDS;
TNF-alpha;
sTNF-RI;
Asthma
- MeSH:
Asthma*;
Cytokines;
Humans;
Immunoassay;
Infant*;
Infant, Newborn;
Inflammation;
Interleukin-1;
Interleukin-6;
Pneumonia;
Prospective Studies*;
Receptors, Tumor Necrosis Factor*;
Tumor Necrosis Factor-alpha*
- From:Pediatric Allergy and Respiratory Disease
1999;9(3):280-289
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Many cytokines including tumor necrosis factor-alpha (TNF-alpha), IL-6, IL-1 influence the inflammatory response in acute and chronic respiratory inflammation. Tumor necrosis factor-alpha is a pleiotropic mediator of many inflammatory responses. Soluble TNF receptor acts in a antiinflammatory manner by inhibit the function of TNF. In order to investigate the role of TNF-alpha and sTNF-RI to develop the chronic airway inflammation in RDS and asthma, We measured the serum levels of TNF-alpha and sTNF-RI in the patients with respiratory distress syndrome, bronchial asthma and normal healthy controls. METHODS: Total 24 subjects (10 of RDS neonates, 6 of health newborn infants, and 8 of infantile asthma) who admitted to our pediatric department of Soonchunhyang university were enrolled to our study. The serum levels of TNF-alpha and soluble sTNF-RI were measured by EIA kit (Quantikine human TNF-alpha Immunoassay, Catalog number DTA50, and Quantikine human sTNF RI Immunoassay, Catalog Number DRT100, R&D). RESULTS: The serum levels of TNF-alpha in the patients with respiratory distress syndrome, bronchial asthma, and normal healthy controls were 4.96+/-3.32 pg/mL, 5.65+/-5.85 pg/mL, 2.95+/-2.33 pg/mL (mean+/-SD), respectively. There was no statistically significance between each group (P=0.732, P=0.367, P=0.255). The serum levels of soluble sTNF-RI in the patients with respiratory distress, bronchial asthma, and normal healthy controls were 156.1+/-166.4 pg/mL, 120.3+/-134.1 pg/mL, 33.3+/-10.7 pg/mL, respectively. There was no statistically significance between each group (P=0.469, P=0.102, P=0.326). There were significant correlation between TNF-alpha and sTNF-RI in the group of normal healthy controls (r=0.9534, P=0.0032,). But, there was no significant correlation between TNF-alpha and sTNF-RI in the group of respiratory distress syndrome (r=0.5940, P=0.0702). CONCLUSIONS: In this study, it is suggested that disturbance of the ratio of TNF-alpha to sTNF-RI play a role as a factor to persist pulmonary inflammation which lead to a development of bronchial asthma.
