Molecular characteristics and drug resistance phenotype of Salmonella enteritidis in patients with diarrhea in Xiangyang
10.3969/j.issn.1006-2483.2024.06.019
- VernacularTitle:襄阳市腹泻患者肠炎沙门菌分子特征及耐药表型分析
- Author:
Jing SHI
1
;
Yating ZHANG
2
;
Shengming ZHU
1
;
Ziyu YANG
1
;
Gang YANG
1
Author Information
1. Xiangyang Center for Disease Control and Prevention , Xiangyang , Hubei 430074 , China
2. Hubei Provincial Center for Disease Control and Prevention , Wuhan , Hubei 430079 , China
- Publication Type:Journal Article
- Keywords:
Salmonella;
Molecular typing;
Drug resistance;
Whole genome sequencing
- From:
Journal of Public Health and Preventive Medicine
2024;35(6):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the serotype, drug resistance, and molecular typing characteristics of Salmonella enteritidis strains isolated from patients with diarrhea in Xiangyang City. Methods Serotyping, drug sensitivity test, pulsed field gel electrophoresis (PFGE) typing, and whole genome sequencing were carried out on Salmonella enteritidis strains isolated from diarrhea patients from 2019 to 2020. Results From the drug susceptibility test , Salmonella enteritidis in this region showed varying degrees of drug resistance, with 94.7% of Salmonella enteritidis strains being multidrug-resistant. The PFGE electrophoretogram of Salmonella enteritidis showed that 19 strains could be divided into 6 PFGE types by cluster analysis, among which 12 strains belonged to the epidemic dominant type. MLST typing showed that all 15 strains were ST11 type, carrying multiple drug resistance genes (mdsA, mdsB, mdsC, gols, and sdiA) and mdtK gene, and all carried various invasiveness-related virulence genes. However, there were slight differences in virulence genes related to the secretion system of the pathogenic island among the different strains. The phylogenetic tree analysis of the system evolution showed that 15 strains could be divided into 2 different evolutionary branches. Conclusion The situation of multidrug resistance in Salmonella enteritidis in Xiangyang is serious, with dominant PFGE types and ST types, and it carries multiple drug resistance genes and various invasiveness-related virulence genes.
