Antioxidant activity of water extract from bamboo stems and its protective effect on t-BHP induced oxidative damage in Caco-2 cells
10.3969/j.issn.1006-2483.2024.06.011
- VernacularTitle:楠竹茎水浸提物抗氧化活性及对t-BHP诱导Caco-2细胞氧化损伤的保护作用
- Author:
Xin YUAN
1
,
2
;
Yunlong HUANG
2
;
Xiaomin XIE
1
,
2
;
Zihan ZHONG
2
;
Jiarui CHEN
2
;
Cuiyu BAO
2
;
Xu YANG
3
,
4
;
Ping MA
2
,
3
Author Information
1. School of Pharmacy , Hubei University of Science and Technology , Xianning , Hubei 437100 , China
2. School of Basic Medical Sciences , Hubei University of Science and Technology , Xianning , Hubei 437100 , China
3. Hubei Industrial Technology Research Institute of Intelligent Health，Xianning , Hubei 437100 , China
4. Institute of Natural Antioxidants and Anti-inflammation , Dali University , Dali , Yunnan 671003 , China
- Publication Type:Journal Article
- Keywords:
Bamboo stem;
Free radical scavenging rate;
Caco-2 cells;
Tert butyl hydrogen peroxide(t-BHP)Incidence rate;
Antioxidant activity
- From:
Journal of Public Health and Preventive Medicine
2024;35(6):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antioxidant activity of bamboo stem extracts and the therapeutic effect of bamboo stem water extract on oxidative inflammation induced by tert butyl hydroperoxide (t-BHP) in human colon adenocarcinoma cells (Caco-2). Methods In this study, ABTS, DPPH, and FRAP assays were used to determine the extracellular antioxidant activity of petroleum ether extract, ethyl acetate extract, n-butanol extract, 95% ethanol extract, and distilled water extract from bamboo stems. The human intestinal Caco-2 cell line was used as the model cell, and t-BHP was selected as the oxidative stress modeling agent. The CCK-8 assay was used to detect cell viability and the optimal oxidative damage concentration of t-BHP. The content of MDA, 8-OHdG, TNF-α and IL-1β were detected to assess antioxidant stress effect. Results The five extracts of bamboo all had certain antioxidant activity, among which the water extract of bamboo stem had the best comprehensive antioxidant activity with high cell viability in Caco-2 cells. The optimal modeling concentration of t-BHP was 200 μMol/L. The water extract of bamboo stem significantly reduced the content of oxidative stress related biomarkers and inflammatory factors in Caco-2 cells induced by t-BHP. Conclusion The stem extracts of bamboo in Xianning City have strong in vitro antioxidant activity. Among them, the water extract of bamboo stem has a protective effect on t-BHP induced oxidative damage in Caco-2 cells, suggesting that the water extract possesses a potential to be developed as new antioxidant products for clinical prevention and treatment of oxidative damage related diseases.
