Effects of antibiotics on energy metabolism and adipose tissue function in high-fat diet-induced obese mice
10.3969/j.issn.1006-2483.2024.06.002
- VernacularTitle:抗生素对高脂饮食诱导肥胖小鼠能量代谢及脂肪组织功能的影响
- Author:
Yuanjie CUI
1
;
Xinwen BI
2
;
Fan BU
3
,
4
;
Jia CUI
5
,
6
;
Qiuxian LU
5
,
6
;
Fang HE
2
;
Hua YANG
2
;
Ming LI
2
Author Information
1. West China School of Public Health/The Fourth Hospital of West China , Sichuan University, Chengdu 610041, China
2. West China School of Public Health/The Fourth Hospital of West China , Sichuan University, Chengdu 610041, China
3. Sichuan Academy of Medical Sciences • Sichuan Provincial People'
4. s Hospital (Affiliated Hospital of UESTC) , Chengdu 610072 , China
5. Second People'
6. s Hospital of Yibin , Yibin , Sichuan 644000 , China
- Publication Type:Journal Article
- Keywords:
Energy metabolism;
Obesity;
White fat;
Brown fat
- From:
Journal of Public Health and Preventive Medicine
2024;35(6):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of antibiotics and high-fat diet on energy metabolism and the browning of white adipose tissue (WAT) and brown adipose tissue (BAT) in mice, so as to provide new ideas for the possible mechanism of adipose tissue in the prevention and treatment of obesity. Methods A total of 80 10-week-old C57BL/6 male mice were fed with normal diet in the early stage, and the antibiotic gavage group (AG) and antibiotic high-fat group (AFG) were given mixed antibiotics by gavage. The blank group (BG) and the high-fat diet group (FG) were given normal saline intragastric solution for 2 weeks, and after the gavage operation, the FG group and the AFG group were given high-fat diet for obesity modeling, and the BG group and AG group continued to be fed with normal diet for 8 weeks (N=20). After the experiment, each group was injected with β3-adrenergic receptor agonists for 5 days, and the high-fat/ordinary diet remained unchanged. At the end of the experiment, basal metabolic rate (BMR), fasting blood glucose (FBG) and rectal temperature were measured, and feces, blood, subcutaneous white fat, epididymis and brown adipose tissue in the scapular area of mice were collected. The automatic biochemical analyzer was used to determine the blood biochemical indexes; reverse transcription polymerase chain reaction (RT-qPCR) was used to measure the expression of genes related to browning of WAT and BAT adipose tissue, respectively. Real-time quantitative polymerase chain reaction (qPCR) was used to determine the expression of WAT mitochondrial DNA (mt DNA). Results From the 4th week to the end of the experiment, the weight of the AFG group was significantly higher than that of the AG group and significantly lower than that of the FG group (P<0.05). The body weight, organ coefficient, serum TC level, rectal temperature and WAT cell diameter in the AFG group were significantly higher than those in the AG group. The serum levels of FBG, TC and LDL in the AFG group were significantly lower than those in the FG group (P<0.05). The overall BMR(mlO2/h) FG group was significantly higher than that of BG group, and the AFG group was significantly higher than that of AG. BMR per unit body weight (mlO2/h/g) AFG was significantly higher than that of FG group (P<0.05). The expressions of RIP140, PPAR-γ and UCP-1 in BAT in the AFG group were significantly higher than those in the FG group, and the mt DNA copy number of WAT in the AFG group was significantly higher than that in the FG group (P<0.05). Conclusion Antibiotic intervention can up-regulate the expression of brown fat-related genes in high-fat diet mice, increase brown fat activity, increase the relative mitochondrial number of white fat, increase the level of browning of white fat, promote thermogenesis, increase the BMR per unit body weight of adult obese mice, and then improve the overall energy metabolism of the body, and slow down the weight gain induced by high-fat diet to a certain extent.
