Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study.
10.5653/cerm.2017.44.2.90
- Author:
Hyung Jun KIM
1
;
Hye Jin YOON
;
Jung Mi JANG
;
Won Don LEE
;
San Hyun YOON
;
Jin Ho LIM
Author Information
1. Maria Fertility Hospital, Seoul, Korea. blue@mariababy.com
- Publication Type:Original Article
- Keywords:
In vitro fertilization;
Insemination;
Intracytoplasmic sperm injection;
Time-lapse imaging
- MeSH:
Blastocyst;
Embryonic Development*;
Embryonic Structures*;
Female;
Fertilization;
Fertilization in Vitro;
Humans*;
In Vitro Techniques*;
Insemination;
Methods;
Oocytes*;
Ovum;
Pregnancy;
Sperm Injections, Intracytoplasmic;
Spermatozoa*;
Time-Lapse Imaging
- From:Clinical and Experimental Reproductive Medicine
2017;44(2):90-95
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination. RESULTS: No significant differences were observed between the two groups regarding clinical outcomes (p>0.05). ICSI-derived embryos showed significantly faster morphokinetics than those derived from conventional IVF, from the time to pronuclear fading to the time to 6 cells (p<0.05). However, no significant differences were found from the time to 7 cells to the time to expanded blastocyst (p>0.05). There were no differences in abnormal cleavage events between the two groups (p>0.05); they showed the same rates of direct cleavage from 1 to 3 cells, 2 multinucleated cells, 2 uneven cells, and reverse cleavage. CONCLUSION: The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. However, these differences did not affect the clinical outcomes of the embryo. Additionally, no significant differences in abnormal cleavage events were found according to the fertilization method.
