Lectin histochemistry in the small intestines of piglets naturally infected with porcine epidemic diarrhea virus
- Author:
Bohye KIM
1
;
Sungwoong JANG
;
Hyewon JANG
;
Joong-Sun KIM
;
Tae-Il JEON
;
Jun-Gyu PARK
;
In-Sik SHIN
;
Kyoung-Oh CHO
;
Changjong MOON
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(5):e66-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This work aimed to examine the alterations in glycoconjugates in the small intestines of piglets naturally infected with PEDV using lectin histochemistry.
Methods:Six piglets including three PEDV-infected and three non-infected piglets were evaluated. Small intestinal samples were histopathologically examined, and lectin histochemistry was performed.
Results:Piglets infected with PEDV had significant histological abnormalities in their small intestines, such as pronounced villous atrophy, varying degrees of villous fusion, and diverse mucosal alterations. Specific regions of the duodenum, jejunum, and ileum showed discernible variations in glycoconjugate distribution, as determined by lectin histochemistry.Compared with the controls, the PEDV-infected piglets showed significant changes in N-acetylglucosamine- and galactose-binding lectins (particularly wheat germ agglutinin and Arachis hypogaea (peanut) agglutinin) in multiple intestinal regions.
Conclusions:and Relevance: These findings can enhance understanding of how viruses such as PEDV impact the glycoconjugate composition of the small intestines and emphasize the potential connection between the pathogenesis of PEDV and glycoconjugate.
