Determining genetic diversity of Microbiology prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India
- Author:
Akash GOLAVIYA
1
;
Rafiyuddin MATHAKIYA
;
Subhash JAKHESARA
;
Prakash KORINGA
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(4):e55-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objectives:This study examined the prevalence and molecular characterization of BRVA in neonatal calves in Gujarat, India.
Methods:Sixty-nine diarrheic fecal samples were collected and subjected to various molecular methods of BRVA detection, isolation, and characterization.
Results:The latex agglutination test (LAT), electropherotyping (RNA-PAGE), and reverse transcription polymerase chain reaction revealed positivity rates of 39.13%, 20.30%, and 37.70%, respectively. RNA-PAGE identified 11 bands with a 4:2:3:2 migration pattern, indicative of the segmented genome of BRVA. BRVA was successfully isolated from LATpositive samples, with 26 samples exhibiting clear cytopathic effects upon passage in MA-104 cell lines. Genotyping identified G10 as the predominant G genotype, with P[11] genotypes comprising 76.92% of the isolates. The most common G/P combination was G10P[11], highlighting its zoonotic potential.
Conclusions:and Relevance: These findings underscore the importance of molecular detection and genotyping for effective vaccine development. This study provides crucial insights into the prevalent G and P genotypes of BRVA in Gujarat, India, aiding in the development of targeted control measures.
