Determination of three forms of mercury in human hair by high performance liquid chromatography-atomic fluorescence spectrometry
10.20001/j.issn.2095-2619.20240815
- VernacularTitle:高效液相色谱-原子荧光光谱法测定人发中3种形态汞
- Author:
Haiyan YI
1
;
Yunfeng NIE
;
Qiang XI
;
Lu YANG
Author Information
1. Hunan Prevention and Treatment Institute for Occupational Disease, Changsha, Hunan 410007, China
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Atomic fluorescence spectrometry;
Human hair;
Mercury;
Methylmercury;
Ethylmercury
- From:
China Occupational Medicine
2024;51(4):434-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of mercury, methylmercury (MeHg) and ethylmercury (EtHg) in the hair of mercury-exposed individuals using high performance liquid chromatography-atomic fluorescence spectrometry. Methods About 0.20 g of hair was mixed with 5.0 mL of hydrochloric acid to a concentration of 5.00 mol/L. After standing for 12 hours, ultrasonic extraction was carried out for 30 minutes at room temperature. Ammonia solution was added dropwise into 2.0 mL supernatant after centrifuge to adjust the pH to 4-5. The solution was diluted to 10.0 mL with pure water after mixed with 0.2 mL of L-cysteine with a mass concentration of 10.0 g/L. The mobile phase consisted of 5.0% methanol + a mass concentration of 0.06 mol/L ammonium acetate + a 0.1% L-cysteine, and was used for separation by a C18 liquid chromatography column, followed by detection using atomic fluorescence spectrometry after UV digestion. Results The linear relationship of mercury, MeHg and EtHg in human hair was good at mass concentrations of 0.0-10.0 μg/L, with all correlation coefficients >0.999 0. The detection limit of mercury, MeHg and EtHg was 0.008, 0.008, and 0.010 μg/g, respectively. The within- and between- run relative standard deviations of the three forms of mercury were 1.6%-4.3% and 2.0%-5.6%, respectively, and the recovery rates were 94.0%-101.2%. Conclusion This method is simple, with low detection limit, high sensitivity, and good accuracy, and is suitable for the simultaneous determination of mercury, MeHg and EtHg in the hair of mercury-exposed individuals.
