Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels.
10.3341/jkos.2007.48.11.1548
- Author:
Jae Woo KIM
1
;
Woo Seok CHANG
;
Su Yoon LEE
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Collagen gel;
Contraction;
Nitric oxide;
Trabecular meshwork
- MeSH:
Collagen*;
Gels*;
Humans;
Nitric Oxide*;
Nitroprusside;
S-Nitroso-N-Acetylpenicillamine;
Tissue Donors;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2007;48(11):1548-1553
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs). METHODS: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively. RESULTS: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05). CONCLUSIONS: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.
