Free Cancer Cell Detection in Peritoneal Cavitr of Gastric Cancer Patients by RT-PCR for CEA.
- Author:
See Young KIM
1
;
Jong Inn LEE
;
Nan Mo MOON
;
Nam Sun PAIK
;
Dong Wook CHOI
;
Ho Yoon BANG
;
Woo Chul NOH
Author Information
1. Department of General Surgery, Korea Cancer Center Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Peritoneal seeding;
RT-PCR;
CEA;
Cytology
- MeSH:
Adenocarcinoma;
Humans;
Peritoneal Cavity;
Polymerase Chain Reaction;
Recurrence;
Stomach Neoplasms*
- From:Journal of the Korean Cancer Association
1999;31(1):24-30
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study is aimed to evaluate the efficacy of a novel method (RT-PCR for CEA) to diagnose free cancer cells in peritoneal cavity of gastric cancer patients, which can be used as a indication of prophylactic treatment to prevent peritoneal recurrence after curative resection of gastric cancer. MATERIALS AND METHODS: 114 gastric adenocarcinoma patients were included for this study. With pellet of peritoneal washing fluid, cytology and RT-PCR for CEA were performed with specific primers. RESULTS: Positive rate of PCR as a whole was 55.3% (63 cases); however, that of cytology was 15.8% (18 cases). Positive rate of PCR increased with depth of invasion of the lesion (p=0.026); however, that of cytology didn't (p=0.233). In early gastric cancer and seeding cases, PCR was not more sensitive than cytologic examination in detection of free cancer cell, but in pm, ss and si cancers, PCR was much more sensitive than cytology (p<0.001). CONCLUSION: PCR was more sensitive to diagnose free cancer cells in peritoneal cavity of gastric cancer patients especially in pm, ss and si cancers than conventional cytologic examination, and it can be a good candidate of indication of prophylactic treatment to prevent peritoneal recurrence after curative resection.
