Effects of natural eggshell membrane (NEM) on monosodium iodoacetate-induced arthritis in rats.
10.4163/jnh.2015.48.4.310
- Author:
Boo Yong SIM
1
;
Ji Won BAK
;
Hae Jin LEE
;
Ji Ae JUN
;
Hak Joo CHOI
;
Chang Ju KWON
;
Hwa Young KIM
;
Kevin J RUFF
;
Karsten BRANDT
;
Dong Hee KIM
Author Information
1. Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University, Daejeon 300-176, Korea. dhkim@dju.kr
- Publication Type:Original Article
- Keywords:
osteoarthritis;
anti-Inflammatory;
NEM;
MIA;
health food
- MeSH:
Animals;
Arthritis*;
Cartilage;
Cytokines;
Dinoprostone;
Enzyme-Linked Immunosorbent Assay;
Food, Organic;
Interleukin-6;
Leukotriene B4;
Membranes*;
Osteoarthritis;
Patella;
Rats*;
Synovial Membrane;
Tissue Inhibitor of Metalloproteinase-1
- From:Journal of Nutrition and Health
2015;48(4):310-318
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study is to investigate anti-arthritis activity using natural eggshell membrane (NEM). METHODS: NEM was administered at 52 mg/kg, 200 mg/kg, and 400 mg/kg to SD-Rat, where arthritis was induced by monosodium iodoacetate (MIA) at 3 mg. NO production in serum was measured using Griess reagent. Cytokines including IL-1beta, and IL-6 were measured by Luminex and PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP were measured by ELISA. The cartilage of patella volume was examined and 3-D high-resolution reconstructions of the cartilage of patella were obtained using a Micro-CT system. RESULTS: Production of NO, IL-1beta, IL-6, PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP in serum was decreased, respectively, in comparison with control. The cartilage of patella volume increased significantly. In addition, the NEM group showed a decrease in the cartilage of patella, synovial membrane, and transformation of fibrous tissue. CONCLUSION: The results for NEM showed significant anti-arthritis activity. These results may be developed as a raw material for new health food to ease the symptoms mentioned above.
