Comparative proteomic analysis of Virology PK-15 cells infected with wild-type strain and its EP0 gene-deleted mutant strain of pseudorabies virus
- Author:
Di WANG
1
;
Dongjie CHEN
;
Shengkui XU
;
Fang WEI
;
Hongyuan ZHAO
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(4):e54-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This study examined the function of EP0 to provide a direction for its in-depth analysis.
Methods:In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tagbased proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.
Results:This study identified 7,391 DEPs, including 120 and 21 up-regulated and downregulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.
Conclusions:and Relevance: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.
