In vitro immunoregulatory role of recombinant Ancylostoma ceylanicum calreticulin
- Author:
Tingting ZHUANG
1
;
Asmaa M. I. ABUZEID
;
Xiaoyu CHEN
;
Shilan ZHU
;
Guoqing LI
Author Information
- Publication Type:Original Article
- From: Parasites, Hosts and Diseases 2024;62(1):75-84
- CountryRepublic of Korea
- Language:English
- Abstract: Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host’s immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.
