Investigating the Immune-Stimulating Potential of β-Glucan from Aureobasidium pullulans in Cancer Immunotherapy
10.4062/biomolther.2024.047
- Author:
Jae-Hyeon JEONG
1
;
Dae-Joon KIM
;
Seong-Jin HONG
;
Jae-Hee AHN
;
Dong-Ju LEE
;
Ah-Ra JANG
;
Sungyun KIM
;
Hyun-Jong CHO
;
Jae-Young LEE
;
Jong-Hwan PARK
;
Young-Min KIM
;
Hyun-Jeong KO
Author Information
1. Department of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea
- Publication Type:Original Article
- From:Biomolecules & Therapeutics
2024;32(5):556-567
- CountryRepublic of Korea
- Language:English
-
Abstract:
β-glucan, a polysaccharide found in various sources, exhibits unique physicochemical properties, yet its high polymerization limits clinical applications because of its solubility. Addressing this limitation, we introduce PPTEE-glycan, a highly purified solubleβ-1,3/1,6-glucan derived from Aureobasidium pullulans. The refined PPTEE-glycan demonstrated robust immune stimulation in vitro, activated dendritic cells, and enhanced co-stimulatory markers, cytokines, and cross-presentation. Formulated as a PPTEE + microemulsion (ME), it elevated immune responses in vivo, promoting antigen-specific antibodies and CD8+ T cell proliferation.Intratumoral administration of PPTEE + ME in tumor-bearing mice induced notable tumor regression, which was linked to the activation of immunosuppressive cells. This study highlights the potential of high-purity Aureobasidium pullulans-derived β-glucan, particularly PPTEE, as promising immune adjuvants, offering novel avenues for advancing cancer immunotherapy.
