Prevalence and molecular characteristics of carbapenemresistant Escherichia coli isolated from dogs in South Korea
- Author:
Bo-Youn MOON
1
;
Md. Sekendar ALI
;
Seunghoe KIM
;
Hee-Seung KANG
;
Ye-Ji KANG
;
Jae-Myung KIM
;
Dong-Chan MOON
;
Suk-Kyung LIM
Author Information
- Publication Type:07
- From:Journal of Veterinary Science 2024;25(5):17-2024
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.
Methods:E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).
Results:In total, 13 CRE isolates (0.13%) were identified from dogs possessing blaNDM-5 along with β-lactamase genes, mostly blaCMY-2 (92.2%) and blaTEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried nonbeta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1–P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.
Conclusions:and Relevance: This investigation provides significant insight into the prevalence and molecular characteristics of blaNDM-5 -carrying E. coli in dogs. The co-existence of blaNDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.
