Immunoassay of Pertussis According to Ages.
- Author:
Soo Young LEE
;
Ui Yoon CHOI
;
Ju Sang KIM
;
Joong Hyun AHN
;
Jung Hyun CHOI
;
Sang Hyuk MA
;
Joon Soo PARK
;
Hwang Min KIM
;
Jin Han KANG
- Publication Type:Original Article
- Keywords:
Pertussis;
Immunoassay;
Immunization;
Booster
- MeSH:
Adult;
Antibodies;
Child;
Enzyme-Linked Immunosorbent Assay;
Hospitals, University;
Humans;
Immunization;
Immunoassay;
Korea;
Pertussis Toxin;
Whooping Cough
- From:Korean Journal of Pediatric Infectious Diseases
2012;19(2):55-60
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. METHODS: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers > or =24.0 EU/mL were considered to seroprotective as the manufacturer's protocol. RESULTS: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was 56.16+/-50.54 EU/mL. Among seven age groups, age group <11 year showed the highest GMC (64.78+/-53.24 EU/mL) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). CONCLUSIONS: We found that adolescences or adults (age group > or =11 year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).
