Effect of Nitric Oxide on the Survival of R28 Cells.
10.3341/jkos.2009.50.6.919
- Author:
Jeong Il LEE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Neurotoxicity;
Nitric oxide;
R28 cells
- MeSH:
Culture Media, Serum-Free;
NG-Nitroarginine Methyl Ester;
Nitric Oxide;
Vimentin
- From:Journal of the Korean Ophthalmological Society
2009;50(6):919-922
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the effect of nitric oxide (NO) on the survival of R28 cells. METHODS: After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine (SNAP) at various concentrations, with and without the NO inhibitor, Nomega-Nitro-L-arginine methyl ester (L-NAME) for 1 and 3 days. Cellular survival of R28 cells and the production of NO were quantified by rapid colorimetric assays using the MTT and Griess assay, respectively. To evaluate the effect of serum, 10% serum or serum-free media were used separately. RESULTS: R28 cells showed strong immunoreactivity to GFAP and vimentin compared to S-100 or neurofilament. SNAP inhibited the survival of R28 cells in a dose-dependent manner, and this effect of NO on the cellular survival was abolished by L-NAME. These results were similar after exposure for 1 and 3 days, regardless of the presence of serum in the media. CONCLUSIONS: The current results suggest that NO decreased the survival of R28 cells. Further studies are necessary to evaluate the mechanism of cytotoxicity of the R28 cells.
