Cloning and Sequencing of the phoA Gene which is Regulated by the phoP-phoQ operon in Pathogenic Enteric Bacteria.
10.12701/yujm.1995.12.2.237
- Author:
Sung Kwang KIM
;
Tae Yoon LEE
- Publication Type:Original Article
- Keywords:
Klebsiella pneumoniae;
phoA gene;
Cloning;
Sequencing;
Sequence similarity
- MeSH:
Base Sequence;
Clone Cells*;
Cloning, Organism*;
DNA;
Enterobacteriaceae*;
Escherichia coli;
Klebsiella pneumoniae;
Operon*
- From:Yeungnam University Journal of Medicine
1995;12(2):237-245
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The DNA fragment containing the phoA of Klebsiella pneumoniae was cloned into pACYC184. The size of the insert. was 4.0 kb and the restriction map showed it contained 3 Pstl sites and 4 PvuLI sites. The nucleotide sequence of the phoA region was determined, which showed strong (80%) sequence similarity with that of Escherichia coli. This suggested that these two species are phylogenetically very close to each other.
