Effect of Baclofen on the Cholinergic Nerve Stimulation in Isolated Rat Detrusor.
10.12701/yujm.1995.12.2.246
- Author:
Kwang Youn LEE
;
Keun Mi LEE
;
Eun Mee CHOI
;
Hyoung Chul CHOI
;
Jeoung Hee HA
;
Won Joon KIM
- Publication Type:Original Article
- MeSH:
Acetylcholine;
Adenosine;
Animals;
Atropine;
Baclofen*;
Decapitation;
Exsanguination;
Hydrogen-Ion Concentration;
Myography;
Rats*;
Receptors, Muscarinic
- From:Yeungnam University Journal of Medicine
1995;12(2):246-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study aimed to investigate the mechanism of action of baclofen on the detrusor muscle isolated from rat. Rats (Sprague-Dawley) were sacrificed by decapitation and exsanguination. Horizontal muscle strips of 2 mm x 15mm were prepared for isometric myography in isolated muscle chamber bubbled with 95% / 5%-OZ / CO2 at 371C, and the pH was maintained at 7.4 Detrusor strips. contracted responding to the.. electrical field stimulation (EFS) by 2 Hz, 2U msec, monophasic square wave of 60 VDC. The initial peak of EFS-Induced contraction was tended to be suppresed by a,p-methylene-adenosine 5'-triphosphate (mATP), a partial agonist of purinergic receptor, and baclofen, a GABAB receptor agonist (statistically nonsignificant). The late sustained contraction by EFS was suppressed significantly (p < 0.05) by additions of atropione, a cholinergic muscarinic receptor antagonist and baclofen. The adenosine 5'-triphosphate-induced contraction was completely abolished by mA TP but not by baclofen. In the presence of atropine, the subsequent addition of acetylcholine could not contract the muscle strips: but the addition of acetylcholine in the presence of baclofen evoked a contraction to a remarkable extent.
