Simultaneous determination of five flavonoids in Ganmao'an granules by HPLC-MS/MS
10.12206/j.issn.2097-2024.202403030
- VernacularTitle:HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量
- Author:
Fangjian CHEN
1
,
2
;
Jinqian LUO
;
Zhijun WANG
;
Yeshuai HU
;
Yuxin SUN
;
Hongjie SONG
Author Information
1. 海军军医大学第一附属医院药剂科,上海 200433
2. 黄浦区老年护理医院药剂科,上海 200010
- Keywords:
HPLC-MS/MS;
Ganmao'an granules;
rutin;
hyperin;
isoquercitrin;
quercetin;
luteolin
- From:
Journal of Pharmaceutical Practice and Service
2024;42(9):402-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous determination of five flavonoids in Ganmao'an granules(GMA).Methods Chromatographic separation was achieved on a Kromasil C18 Column(150 mm×4.6 mm,5 μm,100 ?),which was eluted with methanol(A)-0.1％formic acid(B)at the flow rate of 1.0 ml/min.The gradient condition was as follows:0-20 min,35％A,and 20-40 min,45％A.The column temperature was 25 ℃.Analytes were detected using a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source in the negative ion scanning.The multiple reaction monitoring mode was used for qualitative analysis.For each flavonoid,two precursor ion/product ion transitions were chosen:lutin m/z 609.1→300.1,hyperin and isoquercitrin m/z 463.0→300.1,quercetin m/z 301.0→151.0,luteolin m/z 285.0→132.9.Results Five flavonoids showed the good relationships within their own concentration ranges(correlation coefficient r>0.999 1),whose average recoveries were in the range of 100.63％-102.81％with RSDs of 0.67％-2.07％.The content results of rutin,hyperoside,isoquercetin,quercetin,and luteolin in 10 batches of GMA were 32.23-479.83,0.291-1.825,11.44-20.54,6.32-18.41,3.46-6.51 μg/g,respectively.Conclusion The results indicated that the developed method was sensitive,accurate and could provide excellent specificity for simultaneous determination of five flavonoids in GMA.
