Determination of phosphorus content in calcium hydrogen phosphate tablets by 31P-qNMR
10.12206/j.issn.2097-2024.202404063
- VernacularTitle:核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量
- Author:
Zhewei XIA
1
;
Yuanye ZENG
;
Haifei ZHU
;
Yu LI
;
Xiaofei CHEN
Author Information
1. 海军军医大学药学系,上海 200433
- Keywords:
31P-NMR;
q-NMR;
content determination;
calcium hydrogen phosphate
- From:
Journal of Pharmaceutical Practice and Service
2024;42(9):399-401,406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high-precision and high-specificity quantitative nuclear magnetic resonance phosphorus spectrometry method(31P-qNMR)for detecting the phosphorus content in calcium hydrogen phosphate chewable tablets.Methods Hexamethyl phosphoramide was used as an internal standard,phosphorus spectrum data of calcium hydrogen phosphate chewable tablets solution was collected.The linear relationship of calcium hydrogen phosphate within the concentration range of 1.0 to 9.0 mg/ml was studied,and sample recovery rate and repeatability experiments were conducted.Results Calcium hydrogen phosphate and the internal standard demonstrated a good linear relationship within the concentration range of 1.0 to 9.0 mg/ml,with a regression equation A=0.004 3+0.183 6C.The recovery rates for low,medium,and high concentrations were 98.0%,98.1%,and 98.4%,respectively,with relative standard deviations(RSD)of 0.31%,0.68%,and 0.62%,respectively(n=9).The average content of calcium hydrogen phosphate in the chewable tablets was determined to be 98.1%.Conclusion This study successfully established a high-precision and highly specific method for quantifying the content of calcium hydrogen phosphate in chewable tablets,which did not require a self-reference substance,was simple in sample preparation,and had a fast detection speed.The method could provide a new technical support for the quality control of phosphorus-containing drugs.
