Pharmacodynamic effects of Qiwei Zhigan prescription on non-alcoholic steatohepatitis in animal model
10.12206/j.issn.2097-2024.202404096
- VernacularTitle:七味脂肝方对非酒精性脂肪性肝炎动物模型的药效学评价
- Author:
Jianing YANG
1
;
Yiying ZHAO
;
Wei XIAO
Author Information
1. 上海图锋医药科技有限公司,上海 201203
- Keywords:
traditional Chinese medicine;
Qiwei Zhigan prescription;
non-alcoholic steatohepatitis;
methionine-choline deficiency;
choline deficiency
- From:
Journal of Pharmaceutical Practice and Service
2024;42(9):389-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of Qiwei Zhigan prescription on two classic animal models of non-alcoholic steatohepatitis(NASH).Methods Methionine-choline-deficient(MCD)rat model and choline-deficient,L-amino acid-defined,high-fat and high cholesterol diet(CDAHFHC)mouse model were used.48 rats and 96 mice were randomly divided into 6 groups:control group,model group,Qiwei Zhigan prescription group(low,medium and high dose group),positive drug group.After 2 weeks of modeling,the drugs were administrated continuously for 28 days.The efficacy of Qiwei Zhigan prescription in the treatment of non-alcoholic steatohepatitis was evaluated by detecting the serum liver function,blood lipid biochemical index and liver tissue lipid levels of each group of animals,as well as by evaluating the liver histopathological changes.Results The gross anatomical morphology of the liver was improved by Qiwei Zhigan after oral administration of clinical equivalent dose or higher doses in two experimental animal models.The liver weight and liver index of NASH mice were reduced by the prescription.The levels of ALT,ALP and LDH in serum of NASH rats were decreased,and the level of HDL was increased.The levels of ALT,AST,TBIL,ALP,LDH and TG in serum of NASH mice were decreased,and the content of TG in liver was also decreased.The pathological damage of liver tissue was ameliorated,the inflammation of liver tissue was reduced,as well as the degree of ballooning of liver cells and the NAS score of the liver,and there was a certain dose-effect relationship between the groups;the proliferation of collagen fibers in the hepatic lobules and around the portal area of NASH mice was significantly reduced;the density of positive cells labeled by F4/80 was decreased,and the activation of Kupffer cells was alleviated.Conclusion Qiwei Zhigan prescription could effectively ameliorate the progression of two classic NASH models,and decrease the development trend from liver inflammation to liver fibrosis in NASH.
