Establishment of mouse model of hand-foot syndrome induced by capecitabine
10.12206/j.issn.2097-2024.202308045
- VernacularTitle:卡培他滨致小鼠手足综合征模型的建立及评价
- Author:
Peng WANG
1
;
Shun CHEN
;
Yi ZHAO
;
Shouhong GAO
;
Zhipeng WANG
Author Information
1. 海军军医大学第二附属医院药剂科,上海 200003
- Keywords:
capecitabine;
hand-foot syndrome;
animal model;
drug concentration;
ICR mice
- From:
Journal of Pharmaceutical Practice and Service
2024;42(9):385-388,398
- CountryChina
- Language:Chinese
-
Abstract:
Objective Hand-foot syndrome is a dose-limiting toxicity of capecitabine.At present,there is no unified gold standard for the establishment of hand-foot syndrome model.To induce hand-foot syndrome and provide a reference for the establishment of hand-foot syndrome model by administering capecitabine in ICR mice.Methods 42 male ICR mice were randomly divided into control group(6 mice)and experimental group(36 mice).The experimental group was given capecitabine(275 mg/kg,twice/d)by intragastric administration for two weeks,and the control group was given 0.5%CMC-Na(4 ml/kg,twice/d),to evaluate whether the animal model of hand-foot syndrome was successfully constructed through H&E staining of mouse foot skin samples and observe morphological changes and the characteristic appearance of mouse foot skin.After the experiment,the mice were sacrificed,and plasma was collected to quantify the concentrations of capecitabine and metabolites.Results Control mice did not showed symptoms of hand-foot syndrome.The skin of the feet of 19 mice in the experimental group showed symptoms such as erythema and swelling,and H&E staining results showed that the plantar skin angular epidermis was thickened,and part of the keratin was exfoliated and damaged,which was considered to be hand-foot syndrome.There were no significant differences in the concentrations of capecitabine and its metabolites between mice with and without hand-foot syndrome.Conclusion The model of hand-foot syndrome induced by capecitabine in mice was successfully established.Differences in exposure levels of capecitabine and metabolites may not be the cause of hand-foot syndrome.
