Comparison of Methods for Detecting Bacterial Contamination in Platelet Concentrates.
- Author:
So Yong KWON
1
;
Kyoung Young CHOI
;
A Hyun LIM
;
Kyoung Un PARK
;
Nam Sun CHO
Author Information
1. Blood Transfusion Research Institute, Korean Red Cross, Seoul, Korea. sykwon@redcross.or.kr
- Publication Type:Original Article
- Keywords:
Bacterial contamination;
Platelets;
BacT/ALERT 3D system;
Pall eBDS system
- MeSH:
Benzeneacetamides;
Blood Platelets;
Piperidones;
Pneumonia
- From:Korean Journal of Blood Transfusion
2011;22(2):99-109
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Bacterial contamination of platelets represents the highest infectious risk for a transfusion. In this study, we evaluated 2 culture-based systems that have been approved by the US FDA for bacterial screening. METHODS: Platelet concentrates were inoculated with 5 bacterial species to give a final concentration of 10(0), 10(1) and 10(2) CFU/mL. Samples for culture were taken immediately after inoculation (0 hr sample) and after 24 hrs (24 hr sample). For the BacT/ALERT 3D system, a 10 mL sample was inoculated into an aerobic culture bottle and incubated for 7 days. For the Pall eBDS system, 3 mL samples were taken from the 0 hr and 24 hr samples, respectively. The samples were incubated for 24 hrs and 30 hrs. RESULTS: Both systems detected all inoculated units both in the 0 hr and 24 hr samples, except for units inoculated with K. pneumoniae. Eleven units out of 30 units inoculated with K. pneumoniae were detected by the BacT/ALERT 3D system in the 24 hr samples. The Pall eBDS system detected 8 of 30 units in the 24 hr samples. CONCLUSION: Implementation of either system will decrease the risk of transfusing bacterially contaminated platelets. However, testing for bacterial contamination will not completely prevent septic transfusion reactions; pathogen inactivation that is now available should also be considered as an alternative method to reduce the risk of bacterial contamination.
