Study on Chemical Constituents in Thamnolia Subuliformis (Ehrh.) W.Culb. by UHPLC-Q-Exactive Focus-MS/MS
10.13748/j.cnki.issn1007-7693.20223535
- VernacularTitle:基于UHPLC-Q-Exactive Focus-MS/MS的太白茶化学成分研究
- Author:
Shasha LI
1
,
2
;
Zhibiao DI
1
,
2
;
Jing HU
1
,
2
;
Yajuan LI
3
;
Ajuan MAO
1
,
2
;
Fan LI
1
,
2
;
Fang LI
1
,
2
;
Hong ZHANG
1
,
2
;
Weifeng WANG
1
,
2
Author Information
1. Shaanxi Provincial Academy of Traditional Chinese Medicine, Xi&rsquo
2. an 710003, China
3. Shaanxi University of Chinese Medicine, Xianyang 712046, China
- Publication Type:Journal Article
- Keywords:
Thamnolia subuliformis (Ehrh.) W.Culb.;
UHPLC-Q-Exactive Focus-MS/MS;
chemical constituents
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(1):71-79
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE
To conduct a rapid qualitative study on the chemical constituents of the methanol extract in Thamnolia subuliformis (Ehrh.) W.Culb. by UHPLC-Q-Exactive Focus-MS/MS.
METHODS
The chromatographic separation was performed on a Waters Acquity UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) column with acetonitrile(A)-0.1% formic acid aqueous solution(B) as mobile phase for gradient elution, the flow rate was 0.3 mL·min–1 and the column temperature was 30 ℃. Mass spectrometry was performed using an electrospray ionization and data was collected in negative ion modes, and the detection range was m/z 80−1 200. The chemical constituents in Thamnolia subuliformis (Ehrh.) W.Culb. were identified rapidly and comprehensively based on the accurate relative molecular and combined with literature data and reference substances.
RESULTS
A total of 39 chemical constituents were speculatively identified, including 9 mono-substituted phenyl rings, 11 depsides, 5 depsidones, 2 dibenzofuran, 10 lipids, and 2 others.
CONCLUSION
The chemical constituents in the Thamnolia subuliformis (Ehrh.) W.Culb. can be identified accurately and rapidly by this method. Among them, 3 compounds(β-resorcylic acid, usnic acid, atranorin) are unambiguously identified by comparing with reference standards, 19 compounds are found from Thamnolia subuliformis (Ehrh.) W.Culb. for the first time. This paper can provide the important basis for study on pharmacodynamic material and substitute development of Thamnolia subuliformis (Ehrh.) W.Culb..
