Optimization of Extraction Technology and Antioxidant Activity of β-Asarone from Acori Tatarinowii Rhizoma in Vitro
10.13748/j.cnki.issn1007-7693.20222806
- VernacularTitle:石菖蒲中β-细辛醚的提取工艺优化及其体外抗氧化活性研究
- Author:
Yingying YAN
1
;
Manli WANG
1
;
Jinhong LI
1
;
Chenglong LI
1
;
Guanbo HONG
1
;
Liping HUANG
1
,
2
Author Information
1. Lingnan Normal University Mangrove Institute
2. Lingnan Normal University Western Guangdong Characteristic Biology and Medicine Englineering and Research Center, Zhanjiang 524048, China
- Publication Type:Journal Article
- Keywords:
Acori Tatarinowii Rhizoma;
β-asarone;
antioxidant;
orthogonal test;
response surface
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(1):18-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE
To study the best extraction process of β-asarone from Acori Tatarinowii Rhizoma by ethanol heating reflux method, and to explore the antioxidant activity of different segments.
METHODS
With β-asarone from Acori Tatarinowii Rhizoma as the evaluation index to optimize the extraction method. On the basis of a single factor experiment, the effects of ethanol concentration, solid-liquid ratio and extraction time on the extraction amount of β-asarone from Acori Tatarinowii Rhizoma were investigated by orthogonal design and response surface methodology. After the optimal extraction process was determined, the antioxidant activities of different segments were studied.
RESULTS
The optimum extraction process of β-asarone from Acori Tatarinowii Rhizoma was as follows: ethanol concentration was 95%, solid-liquid ratio was 1∶20 g·mL–1 and extraction time was 2.5 h. Under these conditions, the extraction amount of β-asarone from Acori Tatarinowii Rhizoma was 0.918 7 mg·g–1. The results of in vitro antioxidant activity showed that the order of antioxidant capacity was ethyl acetate>petroleum ether>ethanol>n-butanol. Among them, the ethyl acetate fraction had the strongest antioxidant activity, with good ability to scavenge DPPH and ABTS free radicals, and had certain reduction ability.
CONCLUSION
The optimized method is stable, reliable and simple, which can be used for extraction and antioxidant activity determination of β-asarone from Acori Tatarinowii Rhizoma, and provides a basis for the further development of Acori Tatarinowii Rhizoma.
