Effect and Mechanism of Pyridoxine in Alleviating Cisplatin-induced Acute Nephrotoxicity
10.13748/j.cnki.issn1007-7693.20223226
- VernacularTitle:吡哆醇缓解顺铂诱导急性肾毒性的作用及机制
- Author:
Bingbing TANG
1
;
Haichang WANG
1
;
Ling HONG
1
;
Jiajia WANG
1
;
Qinjie WENG
1
Author Information
1. College of Pharmaceutical Sciences, Center for Drug Safety Evaluation and Research, Zhejiang University, Hangzhou 310058, China
- Publication Type:Journal Article
- Keywords:
pyridoxine;
cisplatin;
acute kidney injury;
oxidative stress
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(1):1-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE
To explore whether pyridoxine can protect acute kidney injury caused by cisplatin and to analyze its specific mechanism.
METHODS
After establishing an in vitro model of cisplatin-induced damage in HK-2 cells, different concentrations of pyridoxine were administered and cell survival was detected using SRB, the expression of apoptosis-related and antioxidant proteins were detected by Western blotting, and the activity of reactive oxygen species(ROS) and superoxide dismutase(SOD) were detected by kits. A cisplatin-induced mouse kidney injury model was further established, and the serum urea nitrogen level was detected after the administration of 40 mg·kg–1 pyridoxine treatment, the results of hematoxylin-eosin staining in renal tissue were analyzed, and the expression of NRF2 was detected by Western blotting.
RESULTS
Pyridoxine could protect kidney injury caused by cisplatin in HK-2 cells and mouse in vivo. In HK-2 cells, pyridoxine down-regulated ROS level, up-regulated SOD enzyme activity, and up-regulated the expression of NRF2 and its downstream antioxidant-related gene HO-1. Pyridoxine significantly reduced the level of serum urea nitrogen, repaired kidney tissue damage, and up-regulated the expression of NRF2 in kidney injury mice.
CONCLUSION
Pyridoxine protects against cisplatin-induced kidney injury through enhancing the level of anti-oxidative stress.
