Simultaneous Determination of Six Volatile Components in Honghua Xiaoyao Tablets by GC
10.13748/j.cnki.issn1007-7693.20230329
- VernacularTitle:GC同时测定红花逍遥片中6种挥发性成分含量
- Author:
Hua HUANG
1
;
Ming JIANG
1
;
Weinan HU
1
;
Xinli CHAI
1
;
Liangbi SHAO
2
;
Hongyan XU
2
Author Information
1. Quzhou Institute for Food and Drug Control, Quzhou 324000, China
2. Zhejiang Kangde Pharmaceutical Group Co., Ltd., Quzhou 324000, China
- Publication Type:Journal Article
- Keywords:
Honghua Xiaoyao tablets;
GC;
menthone;
menthol;
2-methoxy-4-vinylphenol;
3-butenyl phthalide;
atractyline;
ligustilide;
content determination
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(23):3245-3250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a method for simultaneous content determination of menthone, menthol, 2-methoxy-4-vinylphenol, 3-butenyl phthalide, atractyline, ligustilide in Honghua Xiaoyao tablets. METHODS The quantitative analysis was carried out on a capillary of HP-5(30 m×250 μm, 0.25 μm) column combined with an oven temperature program; inlet port temperature 250 ℃; detector temperature 280 ℃, split sampling at a split ratio 10∶1. RESULTS The 6 components were completely separated and could be well separated from other components; menthone, menthol, 2-methoxy-4-vinylphenol, 3-butenyl phthalide, atractyline, ligustilide showed a good linear relationship with the chromatographic peak area within the range of 1.37-87.38, 13.67-874.55, 2.05-65.49, 3.35-107.20, 3.49-111.60, 6.95-444.60 μg·mL-1(r≥0.999 0). The average recovery was 97.63%(RSD=3.27%), 94.25%(RSD=1.80%), 97.54%(RSD=2.39%), 103.81%(RSD=1.75%), 94.30% (RSD=2.32%), 98.97%(RSD=2.36%)(n=6), respectively. And its content in 39 batches of Honghua Xiaoyao tablets were determined. CONCLUSION This method is easy to operate, accurate, reliable, and has good repeatability, can be used to supplement the insufficient quality control of Honghua Xiaoyao tablets.
