Determination of Fourteen Fluorescent Whitening Agents in Cosmetics by HPLC-MS/MS
- VernacularTitle:HPLC-MS/MS测定化妆品中14种荧光增白剂
- Author:
Xia WANG
1
;
Liqin LIN
1
;
Zhi YANG
1
;
Liqiong GAO
1
;
Xiuxiu WANG
1
;
Mengna JIN
1
;
Hongjian CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: HPLC-MS/MS; fluorescent whitening agents; cosmetic
- From: Chinese Journal of Modern Applied Pharmacy 2023;40(23):3236-3244
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE To establish a method for the determination of fourteen fluorescent whitening agents in cosmetics by HPLC-MS/MS. METHODS Samples were extracted on a Waters ACQUITY UPLC ®BEH C18(2.1 mm×50 mm, 1.7 μm) column after ultrasonic extracted by DMF with the mobile phase consisted of methanol-0.1% ammonia water solution by gradient elution. The flow rate was 0.3 mL·min-1. The column temperature was 40 ℃. MS was performed using triple quadrupole mass spectrometry. Electrospray ionization source was operated in the positive/negative mode using multiple reaction monitoring scanning mode. RESULTS The results showed that there were good linear relationships for the fluorescent whitening agents in a certain concentration range with correlation coefficients(r) greater than 0.99. The limits of quantification were 0.01-20 μg·g-1 and the limits of detection were 0.004-8 μg·g-1. The average recoveries at three spiked levels were in the range of 85.4%-108.9%, and the relative standard deviation were in the range of 0.3%-7.2%. CONCLUSION The method has high sensitivity, strong specificity, simple and convenient operation, and is suitable for the detection of fourteen fluorescent whitening agents in cosmetics.
