Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS
10.13748/j.cnki.issn1007-7693.20223494
- VernacularTitle:基于UHPLC-Q-TOF-MS/MS分析猪牙皂中化学成分
- Author:
Xiucuan JING
1
;
Junmao LI
1
;
Yulin FENG
1
;
Fei GE
1
;
Min YAO
2
;
Jiqing BAI
1
,
3
,
4
Author Information
1. Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. Jiangxi Institute for Drug Control, Nanchang 330004, China
3. Shaanxi University of Chinese Medicine, Xianyang 712046, China
4. Shaanxi Chinese Medicine Raw Material Quality Testing Technology Service Center, Xianyang 712046, China
- Publication Type:Journal Article
- Keywords:
Gleditsiae Fructus Abnormalis;
zleditsia sinensis;
chemical composition;
UHPLC-Q-TOF-MS/MS
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(23):3225-3235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To Establish a liquid chromatography-mass spectrometry analysis method to comprehensively and quickly identify the chemical components in Gleditsiae Fructus Abnormalis. METHODS The ultra-high liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS) was applied for the composition analysis of Gleditsiae Fructus Abnormalis extract; its mobile phase was 0.1% formic acid(A)-acetonitrile(B) with gradient elution at a flow rate of 0.3 mL·min-1. Accurate ion mass and secondary fragmentation information were obtained in negative ion mode, combined with databases and standard reference for structure elucidation. RESULTS A total of 78 compounds(32 flavonoids, 15 phenolic acids, 22 lignans and 9 chemical constituents of monoterpenoid acids) with 14 potential new compounds were screened. CONCLUSION This study comprehensively analyzes the chemical composition of Gleditsiae Fructus Abnormalis, and provides a basis for the investigation of the pharmacodynamic basis and quality standards of this drug.
