Determination of Cyperenone and α-Cyperone in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics
10.13748/j.cnki.issn1007-7693.20230631
- VernacularTitle:UPLC-MS/MS测定大鼠血浆中香附烯酮和α-香附酮浓度及其药动学研究
- Author:
Chuanhua FENG
1
;
Huiling GUO
2
;
Xiaolin TANG
3
,
4
;
Xiaojuan ZHAO
2
;
Xinlu FAN
2
;
Dekun LIU
2
;
Gang LI
5
Author Information
1. The First Affiliated Hospital of Nanchang University, Nanchang 330000, China
2. Jiangxi University of Chinese Medicine, Nanchang 330100, China
3. Jiangxi Provincial Children#x27
4. s Hospital, Nanchang 330002, China
5. The 908th Hospital of PLA Joint Logistic Support Force, Nanchang 330000, China
- Publication Type:Journal Article
- Keywords:
Cyperus rotundus L.;
pharmacokinetic;
cyperenone;
α-cyperone;
osthenite
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(23):3197-3201
- CountryChina
- Language:Chinese
-
Abstract:
:OBJECTIVE To establish an UPLC-MS/MS method for the determination of the concentrations of cyperenone and α-cyperone in rat plasma and to study the pharmacokinetics. METHODS Gradient elution was carried out on a Phenomennex C18(150 mm×2.0 mm, 3 μm) column with acetonitrile-water as mobile phase. The column temperature was 30 ℃, injection volume was 1 μL, osthenite was used as the internal standard, electrospray ion source and positive ion mode were used. The m/z values of cyperenone, α-cyperone and osthenite were 219.1/135.1, 219.1/111.0 and 245.0/123.0, respectively. The plasma concentrations of cyperenone and α-cyperone were measured, and the main pharmacokinetic parameters were calculated using DAS 2.0 software. RESULTS The linear relationship of cyperenone was good in the range of 10-500 ng·mL-1(r=0.991 0), and the linear relationship of α-cyperone was good in the range of 2.5-300 ng·mL-1(r=0.994 1), RSDs of intra-day precision were less than 9.45%. RSDs of daytime precision were less than 9.09%. The recoveries were greater than 86.79%. After intragastric administration of essential oil extract(20 mg·kg-1) from Cyperus rotundus L. in SD rats. The pharmacokinetic parameters of Cmax, AUC0-∞ and MRT(0-∞) of cyperenone and α-cyperone were (8 862.59±1 106.81)ng·L-1, (7 060.94±774.25)ng·L-1·h, (3.21±0.72)h and (934.69±106.81)ng·L-1, (792.26±74.52)ng·L-1·h, (4.94± 0.82)h, respectively. CONCLUSION The established method can be used for the rapid and accurate determination of the concentration of cyperenone and α-cyperone in plasma, and can be used for the pharmacokinetic study of cyperenone and α-cyperone in rats in vivo.
