Compatibility of Gardeniae Fructus and Rhododendri Mollis Flos on Pharmacokinetics of Rhodojaponin II and Rhodojaponin III in Rat Plasma
10.13748/j.cnki.issn1007-7693.20231377shu
- VernacularTitle:栀子-闹羊花配伍对大鼠血浆中闹羊花毒素Ⅱ和闹羊花毒素Ⅲ的药动学影响
- Author:
Xiao qiong ZHANG
1
;
Ting LI
2
;
Hui yu WANG
3
;
Nan LIU
4
;
Xiao hong GUO
1
Author Information
1. Department of Pharmacy, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China
2. School of Pharmacy, Chongqing University of Medical Sciences, Chongqing 400016, China
3. Shimadzu Enterprise Management(China) Co., Ltd., Beijing Branch, Beijing 100020, China
4. Department of Technology Research and Development, Zhejiang Kangenbei Group Healthcare Products Co., Ltd., Lanxi 321100, China
- Publication Type:Journal Article
- Keywords:
Rhododendri Mollis Flos , Gardeniae Fructus , compatibility , pharmacokinetics , rhodojaponin III , rhodojaponin II
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(24):3352-3359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of compatibility of Gardeniae Fructus and Rhododendri Mollis Flos on the pharmacokinetic behavior of rhodojaponin II and rhodojaponin III of Rhododendri Mollis Flos. METHODS LC-MS/MS of rhodojaponin II and rhodojaponin III in plasma of rats was developed. The method was then applied to study the blood concentrations of rhodojaponin II and rhodojaponin III in rats after oral compatibility administration of Gardeniae Fructus and Rhododendri Mollis Flos and single decoction administration of Rhododendri Mollis Flos, respectively, then their pharmacokinetic parameters were calculated and statistical analysed.
RESULTS:The calibration curve was good linearity(r>0.999) in the range of 1-200 ng·mL-1 for rhodojaponin II and 1-100 ng·mL-1 for rhodojaponin III, the precision of quality control samples was less than 12% and the accuracy was less than 20%. After administration of compatibility of Gardeniae Fructus and Rhododendri Mollis Flos and Rhododendri Mollis Flos alone, the AUC0-t of rhodojaponin II in vivo was(260.44±51.67) and (213.39±59.03)h·ng·mL-1, respectively, and the AUC0-t of rhodojaponin III was (60.97±22.78) and (22.38±5.55)h·ng·mL-1, respectively. Compared with single decoction of Rhododendri Mollis Flos administration group, the T1/2 and MRT(0-t)of the rhodojaponin II were significantly increased, the AUC0-t, T1/2, Tmax and CL of the rhodojaponin III were also significantly rised after administration of compatibility of Gardeniae Fructus and Rhododendri Mollis Flos.
CONCLUSION:After the compatibility of Gardeniae Fructus and Rhododendri Mollis Flos, the rate of absorption and the distribution volume are increased of rhodojaponin III in rats, while the elimination rate has decreased, the T1/2 of rhodojaponin II and rhodojaponin III are extended, but don't affect the absorption rate of rhodojaponin II in rats.
