Serological and genetic analysis of a Bel subtype caused by a novel 29insG mutation:a case report

VernacularTitle:29insG新突变导致Bel亚型的血清学和分子生物学分析1例
Author: Renlong ZHOU ¹ ; Cuiyun ZHANG ; Guoqing HUANG
Author Information

1. 深圳市龙华区中心医院输血科,广东深圳 518000
Keywords: Bel subtype; serology; gene sequencing
From: Chinese Journal of Blood Transfusion 2024;37(10):1201-1205
CountryChina
Language:Chinese
Abstract: Objective To investigate the serological characteristics and molecular mechanism of a case of Bel subtype.Methods Serological method was used to analyze the ABO blood group of the patient,absorption and elution test was used to detect the weak antigen on red blood cells,and molecular biology method was used to analyze the ABO gene sequence of the patient.Results The forward typing of the patient was O type,but the reverse typing was B type.The weak B antigen was confirmed by absorption and elution method.This mutation was not found in ISBT and related literature,and was discov-ered for the first time.The patient's blood type was Bel(29insertG)/O.01.01 based on the sequence of exon 3-7.Conclusion In the case of ABO blood group incompatibility,the combination of serological and molecular biological detection tech-niques can improve the accuracy and reliability of ABO blood group identification.