Comparison of disinfective power according to application order of 70% isopropyl alcohol and 10% povidone-iodine.
10.4097/kjae.2013.65.6.519
- Author:
Sang Su KIM
1
;
Soo Bong YU
;
Joo Duck KIM
;
Sie Jeong RYU
Author Information
1. Department of Anesthesiology and Pain Medicine, Kosin University College of Medicine, Busan, Korea. siejeong@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
Colonization;
Disinfectants;
Isopropyl alcohol;
Povidone-iodine
- MeSH:
2-Propanol*;
Agar;
Disinfectants;
Disinfection;
Forearm;
Healthy Volunteers;
Povidone-Iodine*;
Skin;
Sterilization
- From:Korean Journal of Anesthesiology
2013;65(6):519-524
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Many disinfectants have been used clinically in both single and combination applications, but there have been few studies on disinfective power according to sterilization sequence when using a combination of disinfectants. The purpose of this study was to evaluate the disinfective power of a combination of 70% isopropyl alcohol and 10% povidone-iodine (PVP-I) according to sterilization sequence. METHODS: Two hundred healthy volunteers were recruited. Subjects were disinfected with a combination of 70% isopropyl alcohol and 10% PVP-I on both forearms, in varying sequence. The AP group included disinfections on the left forearm with isopropyl alcohol first followed by 10% PVP-I, while the PA group included disinfections on the right forearm with same disinfectants in reverse order. Skin cultures were obtained using cotton swabs 3 min after application of each disinfectant, and then were inoculated on blood agar plates for bacterial culture. Cultures were incubated at 37degrees C under aerobic conditions for 48 hours. RESULTS: There was no significant difference in the number of positive cultures after the 1st disinfection (AP, 45; PA, 36, P = 0.262) or the 2nd disinfection (AP, 6; PA, 13, P = 0.157), suggesting that there is no relationship between disinfective power and the sequence of the disinfectants used. The number of positive cultures significantly decreased after the 2nd disinfection (P < 0.01), however. CONCLUSIONS: There was no significant difference in disinfective power according to sterilization sequence with 70% isopropyl alcohol and 10% PVP-I in healthy volunteers. The combination of 70% isopropyl alcohol and 10% PVP-I was more effective than disinfection with a single agent regardless of sterilization sequence.