Screening and content determination of differential quality markers in Zingiber officinale mixed and triturated with Schisandra chinensis before and after processing
- VernacularTitle:干姜拌捣五味子临方炮制前后差异质量标志物筛选及其含量测定
- Author:
Pei ZHONG
1
;
Jianglin XUE
1
;
Quan ZHAO
1
,
2
;
Chanming LIU
1
,
2
;
Xiaojing YAN
2
;
Dan SU
3
;
Yonggui SONG
3
;
Tulin LU
4
;
Wei HUANG
1
Author Information
1. Dept. of Pharmacy，Changzhou Hospital Affiliated to Nanjing University of Chinese Medicine，Jiangsu Changzhou 213003，China
2. Traditional Chinese Medicine Processing Technology Inheritance Base，National Administration of Traditional Chinese Medicine，Jiangsu Changzhou 213003，China
3. Jiangxi Provincial Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Brain Diseases with Cognitive Impairment，Jiangxi University of Chinese Medicine，Nanchang 330004，China
4. School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Zingiber officinale;
Schisandra chinensis;
differential Q-Marker;
HPLC;
fingerprint
- From:
China Pharmacy
2024;35(23):2870-2876
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To screen and quantitatively analyze differential quality markers （Q-Marker） in Zingiber officinale mixed and triturated with Schisandra chinensis （ZMTS） before and after processing. METHODS HPLC fingerprints of before processing [Z. officinale complicated with S. chinensis （ZWS）] and after processing （ZMTS） （10 batches each） were established. The differences of Q-Markers before and after processing were screened by the chemical pattern recognition method and Q-Marker “five principles”， and the contents were determined. RESULTS A total of 14 common peaks were identified in the fingerprints of ZWS， 22 common peaks were identified in the fingerprints of ZMTS， and 8 components were identified. Differential Q-Marker were screened by chemical pattern recognition and Q-Marker “five principles”， i. e. 6-gingerol， schisandrol A schisandrol B， 8-gingerol， 10-gingerol， schisandrin A， schisandrin B， schizandrin C. The average contents of the 8 differential Q-Markers in ZMTS were 229.46， 244.48， 39.96， 44.12， 61.17， 47.82， 100.11 and 9.70 μg/g， respectively. The average contents of the 4 differential Q-Markers （6-gingerol， schisandrol A， schisandrol B， 8-gingerol） in ZWS were 112.58， 19.01， 26.74 and 5.98 μg/g， respectively. CONCLUSIONS In this study， the differential Q-Markers before and after ZMTS processing are screened. The contents of the Q-Markers in ZMTS after processing are higher than those before processing.
